ATRX antibody (N-Term)

Catalog No. ABIN6260147

Product Details anti-ATRX Antibody

Target: ATRX (helicase 2, X-linked (ATRX))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATRX antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Specificity: ATRX Antibody detects endogenous levels of total ATRX.

Predicted Reactivity: Bovine,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ATRX, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ATRX

Target: ATRX (helicase 2, X-linked (ATRX))

Alternative Name: ATRX (ATRX Products)

Synonyms: ATR2 antibody, JMS antibody, MRXHF1 antibody, RAD54 antibody, RAD54L antibody, SFM1 antibody, SHS antibody, XH2 antibody, XNP antibody, ZNF-HX antibody, 4833408C14Rik antibody, AI447451 antibody, DXHXS6677E antibody, HP1-BP38 antibody, Hp1bp2 antibody, Hp1bp38 antibody, MRXS3 antibody, Rad54 antibody, Xnp antibody, atrx antibody, rad54 antibody, atrxl antibody, wu:fb26e12 antibody, wu:fb52h08 antibody, wu:fb72g09 antibody, wu:fb94e07 antibody, zgc:66223 antibody, ATRX, chromatin remodeler antibody, alpha thalassemia/mental retardation syndrome X-linked L homeolog antibody, alpha thalassemia/mental retardation syndrome X-linked homolog (human) antibody, ATRX antibody, Atrx antibody, atrx.L antibody, atrx antibody

Background:

Description: Involved in transcriptional regulation and chromatin remodeling. Facilitates DNA replication in multiple cellular environments and is required for efficient replication of a subset of genomic loci. Binds to DNA tandem repeat sequences in both telomeres and euchromatin and in vitro binds DNA quadruplex structures. May help stabilizing G-rich regions into regular chromatin structures by remodeling G4 DNA and incorporating H3.3-containing nucleosomes. Catalytic component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Its heterochromatin targeting is proposed to involve a combinatorial readout of histone H3 modifications (specifically methylation states of H3K9 and H3K4) and association with CBX5. Involved in maintaining telomere structural integrity in embryonic stem cells which probably implies recruitment of CBX5 to telomers. Reports on the involvement in transcriptional regulation of telomeric repeat-containing RNA (TERRA) are conflicting, according to a report, it is not sufficient to decrease chromatin condensation at telomers nor to increase expression of telomeric RNA in fibroblasts (PubMed:24500201). May be involved in telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines. Acts as negative regulator of chromatin incorporation of transcriptionally repressive histone H2AFY, particularily at telomeres and the alpha-globin cluster in erythroleukemic cells. Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus. On the maternal allele, required for the chromatin occupancy of SMC1 and CTCTF within the H19 imprinting control region (ICR) and involved in esatblishment of histone tails modifications in the ICR. May be involved in brain development and facial morphogenesis. Binds to zinc-finger coding genes with atypical chromatin signatures and regulates its H3K9me3 levels. Forms a complex with ZNF274, TRIM28 and SETDB1 to facilitate the deposition and maintenance of H3K9me3 at the 3' exons of zinc-finger genes (PubMed:27029610).

Gene: ATRX

Molecular Weight: 282kDa

Gene ID: 546

UniProt: P46100