APLF antibody (Internal Region)

Catalog No. ABIN6259979

Product Details anti-APLF Antibody

Target: APLF (Aprataxin and PNKP Like Factor (APLF))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APLF antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: APLF Antibody detects endogenous levels of total APLF.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human APLF, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000, IHC: 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for APLF

Target: APLF (Aprataxin and PNKP Like Factor (APLF))

Alternative Name: APLF (APLF Products)

Background:

Description: Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.

Gene: APLF

Molecular Weight: 57kDa

Gene ID: 200558

UniProt: Q8IW19

Pathways: DNA Damage Repair