AP2M1 antibody (Internal Region)
-
- Target See all AP2M1 Antibodies
- AP2M1 (Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1))
-
Binding Specificity
- Internal Region
-
Reactivity
- Human, Mouse, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This AP2M1 antibody is un-conjugated
-
Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Specificity
- AP2M1 Antibody detects endogenous levels of total AP2M1.
- Predicted Reactivity
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human AP2M1, corresponding to a region within the internal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product AP2M1 Primary Antibody
-
-
- Application Notes
- WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
-
- Target
- AP2M1 (Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1))
- Alternative Name
- AP2M1 (AP2M1 Products)
- Synonyms
- AP50 antibody, CLAPM1 antibody, mu2 antibody, Ap50 antibody, Clapm1 antibody, cb34 antibody, Ap2m1 antibody, dpy-23 antibody, sb:cb34 antibody, zgc:55711 antibody, zgc:85653 antibody, wu:fa97a10 antibody, ap2m1-a antibody, ap2m1 antibody, zgc:56643 antibody, AP2M1 antibody, adaptor related protein complex 2 mu 1 subunit antibody, adaptor-related protein complex 2, mu 1 subunit antibody, adaptor-related protein complex 2, mu 1 subunit, a antibody, adaptor related protein complex 2 mu 1 subunit L homeolog antibody, adaptor-related protein complex 2, mu 1 subunit, b antibody, AP-2 complex subunit mu-1 antibody, clathrin coat assembly protein AP50 antibody, clathrin coat assembly protein ap50 antibody, AP2M1 antibody, Ap2m1 antibody, ap2m1a antibody, ap2m1.L antibody, ap2m1b antibody, ap2m1 antibody, cgd8_950 antibody, PY06523 antibody, PVX_118455 antibody, PVX_123590 antibody, CpipJ_CPIJ003697 antibody
- Background
-
Description: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins, it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling (By similarity).
Gene: AP2M1
- Molecular Weight
- 49kDa
- Gene ID
- 1173
- UniProt
- Q96CW1
- Pathways
- EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation, SARS-CoV-2 Protein Interactome
-