ATG4D antibody (C-Term)
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- Target See all ATG4D Antibodies
- ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This ATG4D antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- ATG4D Antibody detects endogenous levels of total ATG4D.
- Predicted Reactivity
- Pig,Horse,Dog
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human ATG4D, corresponding to a region within C-terminal amino acids.
- Isotype
- IgG
- Top Product
- Discover our top product ATG4D Primary Antibody
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anti-Autophagy related 4D Cysteine Peptidase (ATG4D) (AA 441-470), (C-Term) antibody
ATG4D Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB7567 unconjugated
anti-Autophagy related 4D Cysteine Peptidase (ATG4D) (AA 14-43) antibodyATG4D Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated
anti-Autophagy related 4D Cysteine Peptidase (ATG4D) (AA 220-249) antibodyATG4D Reactivity: Human, Mouse WB, IHC (p) Host: Rabbit Polyclonal RB7565 unconjugated
anti-Autophagy related 4D Cysteine Peptidase (ATG4D) (AA 14-43), (N-Term) antibodyATG4D Reactivity: Human, Mouse WB, IHC (p) Host: Rabbit Polyclonal RB7563 unconjugated
anti-Autophagy related 4D Cysteine Peptidase (ATG4D) (AA 381-474) antibodyATG4D Reactivity: Mouse WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Autophagy related 4D Cysteine Peptidase (ATG4D) (AA 42-71), (N-Term) antibodyATG4D Reactivity: Mouse WB, IF, IHC (p) Host: Rabbit Polyclonal RB14788 unconjugated
anti-Autophagy related 4D Cysteine Peptidase (ATG4D) (AA 227-257) antibodyATG4D Reactivity: Human, Mouse WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
anti-Autophagy related 4D Cysteine Peptidase (ATG4D) antibodyATG4D Reactivity: Human WB, IF Host: Mouse Monoclonal 222CT15-4-1 unconjugated
anti-Autophagy related 4D Cysteine Peptidase (ATG4D) (AA 326-356) antibodyATG4D Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB11866 unconjugated
anti-Autophagy related 4D Cysteine Peptidase (ATG4D) (C-Term) antibody (HRP)ATG4D Reactivity: Human WB, IF, ICC Host: Rabbit Polyclonal HRP
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- Application Notes
- WB 1:500-1:1000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))
- Alternative Name
- ATG4D (ATG4D Products)
- Background
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Description: Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Gene: ATG4D
- Molecular Weight
- 53 kDa
- Gene ID
- 84971
- UniProt
- Q86TL0
- Pathways
- Autophagy
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