SMUG1 antibody (Internal Region)

Catalog No. ABIN6259193

Product Details anti-SMUG1 Antibody

Target: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMUG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: SMUG1 Antibody detects endogenous levels of total SMUG1.

Predicted Reactivity: Pig,Bovine,Horse,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human SMUG1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for SMUG1

Target: SMUG1 (Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1 (SMUG1))

Alternative Name: SMUG1 (SMUG1 Products)

Synonyms: SMUG1 antibody, xsmug1 antibody, 1200013B09Rik antibody, A930006H09Rik antibody, C85220 antibody, FDG antibody, HMUDG antibody, UNG3 antibody, single-strand-selective monofunctional uracil-DNA glycosylase 1 antibody, single-strand selective monofunctional uracil DNA glycosylase antibody, single-strand-selective monofunctional uracil-DNA glycosylase 1 L homeolog antibody, SMUG1 antibody, smug1 antibody, smuG1 antibody, smuG antibody, LOC5577584 antibody, CpipJ_CPIJ002767 antibody, Smug1 antibody, smug1.L antibody

Background:

Description: Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.

Gene: SMUG1

Molecular Weight: 32 kDa

Gene ID: 23583

UniProt: Q53HV7

Pathways: DNA Damage Repair