RAD51C antibody (Internal Region)

Catalog No. ABIN6259171

Product Details anti-RAD51C Antibody

Target: RAD51C (DNA Repair Protein RAD51 Homolog 3 (RAD51C))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD51C antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Rad51C Antibody detects endogenous levels of total Rad51C.

Predicted Reactivity: Sheep

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Rad51C, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for RAD51C

Target: RAD51C (DNA Repair Protein RAD51 Homolog 3 (RAD51C))

Alternative Name: RAD51C (RAD51C Products)

Synonyms: RGD1563765 antibody, CG2412 antibody, Dm CG2412 antibody, Dmel\\CG2412 antibody, zgc:101596 antibody, RAD51C antibody, Rad51c antibody, ATRAD51C antibody, F4L23.21 antibody, RAS associated with diabetes protein 51C antibody, R51H3 antibody, Rad51l2 antibody, BROVCA3 antibody, FANCO antibody, RAD51L2 antibody, RAD51 paralog C antibody, Rad51 recombinase D antibody, Rad51 DNA recombinase 3 antibody, RAD51C protein antibody, RAD51 homolog C antibody, RAS associated with diabetes protein 51C antibody, RAD51C antibody, Rad51c antibody, Rad51D antibody, rad51c antibody

Background:

Description: Essential for the homologous recombination (HR) pathway of DNA repair. Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Part of the RAD21 paralog protein complexes BCDX2 and CX3 which act at different stages of the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 seems to act downstream of BRCA2 recruitment and upstream of RAD51 recruitment, CX3 seems to act downstream of RAD51 recruitment, both complexes bind predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway. Involved in RAD51 foci formation in response to DNA damage suggesting an involvement in early stages of HR probably in the invasion step. Has an early function in DNA repair in facilitating phosphorylation of the checkpoint kinase CHEK2 and thereby transduction of the damage signal, leading to cell cycle arrest and HR activation. Participates in branch migration and HJ resolution and thus is important for processing HR intermediates late in the DNA repair process, the function may be linked to the CX3 complex. Part of a PALB2-scaffolded HR complex containing BRCA2 and which is thought to play a role in DNA repair by HR. Protects RAD51 from ubiquitin-mediated degradation that is enhanced following DNA damage. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and XRCC3. Contributes to DNA cross-link resistance, sister chromatid cohesion and genomic stability. Involved in maintaining centrosome number in mitosis.

Gene: RAD51C

Molecular Weight: 50 kDa

Gene ID: 5889

UniProt: O43502

Pathways: DNA Damage Repair