nectin-3 antibody (Internal Region)

Catalog No. ABIN6259135

Product Details anti-nectin-3 Antibody

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This nectin-3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: PVRL3 Antibody detects endogenous levels of total PVRL3.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human PVRL3, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for nectin-3

Target: nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))

Alternative Name: NECTIN3 (NECTIN3 Products)

Synonyms: PVRL3 antibody, CD113 antibody, CDW113 antibody, NECTIN-3 antibody, PPR3 antibody, PRR3 antibody, PVRR3 antibody, 2610301B19Rik antibody, 3000002N23Rik antibody, 4921513D19Rik antibody, AA407785 antibody, AU016832 antibody, AW538082 antibody, nectin cell adhesion molecule 3 antibody, poliovirus receptor-related 3 antibody, Nectin3 antibody, NECTIN3 antibody, PVRL3 antibody, nectin3 antibody

Background:

Description: Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.

Gene: NECTIN3

Molecular Weight: 61 kDa

Gene ID: 25945

UniProt: Q9NQS3

Pathways: Cell-Cell Junction Organization