MHC Class II RT-1B antibody
Reactivity: Rat, Mouse
FACS, IHC (fro), IHC (p), IHC, WB
Host: Mouse
Monoclonal
OX-6
unconjugated
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- Target See all MHC Class II RT-1B products
- MHC Class II RT-1B
- Reactivity
- Rat, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MHC Class II RT-1B antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
- Brand
- IHC-plus™
- Specificity
- Recognizes a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. Reacts with certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. Does not react with the BDIX rat strain. Expression is polymorphic in mice. This product is routinely tested in flow cytometry on rat splenocytes.
- Purification
- Protein G purified
- Immunogen
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Rat thymocyte membrane glycoprotein.
Type of Immunogen: Membrane extract - Clone
- OX-6
- Isotype
- IgG1
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- Application Notes
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Approved: Flo (1:50 - 1:100), IHC, IHC-Fr, IHC-P (10 μg/mL), WB
Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Method sheets are available on request. Immunohistology: This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer pH 6.This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) - Comment
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Target Species of Antibody: Rat
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.2, 0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- MHC Class II RT-1B
- Abstract
- MHC Class II RT-1B Products
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