TSEN54 antibody (Internal Region)

Catalog No. ABIN6258653

Product Details anti-TSEN54 Antibody

Target: TSEN54 (tRNA Splicing Endonuclease 54 (TSEN54))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TSEN54 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: TSEN54 Antibody detects endogenous levels of total TSEN54.

Predicted Reactivity: Bovine

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TSEN54, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for TSEN54

Target: TSEN54 (tRNA Splicing Endonuclease 54 (TSEN54))

Alternative Name: TSEN54 (TSEN54 Products)

Synonyms: zgc:109927 antibody, 0610034P02Rik antibody, PCH2A antibody, PCH4 antibody, SEN54L antibody, sen54 antibody, tRNA splicing endonuclease subunit 54 antibody, TSEN54 tRNA splicing endonuclease subunit antibody, tRNA splicing endonuclease 54 homolog (S. cerevisiae) antibody, TSEN54 antibody, tsen54 antibody, Tsen54 antibody

Background:

Description: Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events.

Gene: TSEN54

Molecular Weight: 59 kDa

Gene ID: 283989

UniProt: Q7Z6J9

Pathways: Synaptic Membrane