CD177 antibody (Internal Region)

Catalog No. ABIN6258621

Product Details anti-CD177 Antibody

Target: CD177 (CD177 Molecule (CD177))

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD177 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity: CD177 Antibody detects endogenous levels of total CD177.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CD177, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Expiry Date: 12 months

Target Details for CD177

Target: CD177 (CD177 Molecule (CD177))

Alternative Name: CD177 (CD177 Products)

Synonyms: HNA-2a antibody, HNA2A antibody, NB1 antibody, NB1 GP antibody, PRV-1 antibody, PRV1 antibody, RGD1562941 antibody, CD177 antibody, MGC157159 antibody, 1190003K14Rik antibody, Pdp3 antibody, CD177 molecule antibody, CD177 antigen antibody, CD177 antibody, Cd177 antibody

Background:

Description: In association with beta-2 integrin heterodimer ITGAM/CD11b and ITGB2/CD18, mediates activation of TNF-alpha primed neutrophils including degranulation and superoxide production (PubMed:21193407). In addition, by preventing beta-2 integrin internalization and attenuating chemokine signaling favors adhesion over migration (PubMed:28807980). Heterophilic interaction with PECAM1 on endothelial cells plays a role in neutrophil transendothelial migration in vitro (PubMed:17580308). However, appears to be dispensable for neutrophil recruitment caused by bacterial infection in vivo (PubMed:23461681). Acts as a receptor for the mature form of protease PRTN3 allowing its display at the cell surface of neutrophils (PubMed:17244676, PubMed:18462208). By displaying PRTN3 at the neutrophil cell surface, may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (PubMed:23202369).

Gene: CD177

Molecular Weight: ?46 kDa

Gene ID: 57126

UniProt: Q8N6Q3