ARID1B antibody (Internal Region)

Catalog No. ABIN6258593

Product Details anti-ARID1B Antibody

Target: ARID1B (AT Rich Interactive Domain 1B (SWI1-Like) (ARID1B))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARID1B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunocytochemistry (ICC)

Specificity: BAF250B Antibody detects endogenous levels of total BAF250B.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human BAF250B, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ARID1B

Target: ARID1B (AT Rich Interactive Domain 1B (SWI1-Like) (ARID1B))

Alternative Name: ARID1B (ARID1B Products)

Synonyms: fd07d11 antibody, si:dkey-60a16.3 antibody, wu:fd07d11 antibody, 8030481M12 antibody, 9330189K18Rik antibody, AI836955 antibody, Ardi1b antibody, B230217J03Rik antibody, mKIAA1235 antibody, 6A3-5 antibody, Smcf1 antibody, smcf-1 antibody, BAF250B antibody, BRIGHT antibody, DAN15 antibody, ELD/OSA1 antibody, MRD12 antibody, OSA2 antibody, P250R antibody, AT rich interactive domain 1B (SWI1-like) antibody, AT rich interactive domain 1B (SWI-like) antibody, AT-rich interaction domain 1B antibody, arid1b antibody, Arid1b antibody, ARID1B antibody

Background:

Description: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Binds DNA non-specifically (PubMed:14982958, PubMed:15170388).

Gene: ARID1B

Molecular Weight: 170 kDa

Gene ID: 57492

UniProt: Q8NFD5