ARFGEF2 antibody (N-Term)

Catalog No. ABIN6258592

Product Details anti-ARFGEF2 Antibody

Target: ARFGEF2 (ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 2 (Brefeldin A-Inhibited) (ARFGEF2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARFGEF2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: ARFGEF2 Antibody detects endogenous levels of total ARFGEF2.

Predicted Reactivity: Pig,Horse,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ARFGEF2, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ARFGEF2

Target: ARFGEF2 (ADP-Ribosylation Factor Guanine Nucleotide-Exchange Factor 2 (Brefeldin A-Inhibited) (ARFGEF2))

Alternative Name: ARFGEF2 (ARFGEF2 Products)

Synonyms: AI463430 antibody, AW493672 antibody, BIG2 antibody, E230011G24Rik antibody, Big2 antibody, PVNH2 antibody, dJ1164I10.1 antibody, ADP ribosylation factor guanine nucleotide exchange factor 2 antibody, ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) antibody, ARFGEF2 antibody, Arfgef2 antibody

Background:

Description: Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extent on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles, the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways.

Gene: ARFGEF2

Molecular Weight: 210 kDa

Gene ID: 10564

UniProt: Q9Y6D5