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USP28 antibody (N-Term)

USP28 Reactivity: Human, Mouse, Rat WB, ELISA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6258349
  • Target See all USP28 Antibodies
    USP28 (Ubiquitin Specific Peptidase 28 (USP28))
    Binding Specificity
    • 15
    • 8
    • 7
    • 7
    • 7
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    N-Term
    Reactivity
    • 40
    • 16
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 52
    • 2
    • 1
    Rabbit
    Clonality
    • 55
    Polyclonal
    Conjugate
    • 17
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This USP28 antibody is un-conjugated
    Application
    • 31
    • 19
    • 13
    • 13
    • 13
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA
    Specificity
    USP28 Antibody detects endogenous levels of total USP28.
    Predicted Reactivity
    Pig,Bovine,Horse,Sheep,Rabbit,Dog
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogen
    A synthesized peptide derived from human USP28, corresponding to a region within N-terminal amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product USP28 Primary Antibody
  • Application Notes
    WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Expiry Date
    12 months
  • Target
    USP28 (Ubiquitin Specific Peptidase 28 (USP28))
    Alternative Name
    USP28 (USP28 Products)
    Synonyms
    im:7138195 antibody, USP28 antibody, 9830148O20Rik antibody, AU022237 antibody, mKIAA1515 antibody, ubiquitin specific peptidase 28 antibody, USP28 antibody, usp28 antibody, Usp28 antibody
    Background

    Description: Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells (PubMed:24623306).

    Gene: USP28

    Molecular Weight
    122 kDa
    Gene ID
    57646
    UniProt
    Q96RU2
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