USP13 antibody (C-Term)

Catalog No. ABIN6258346

Product Details anti-USP13 Antibody

Target: USP13 (Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity: USP13 Antibody detects endogenous levels of total USP13.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human USP13, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IHC: 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for USP13

Target: USP13 (Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13))

Alternative Name: USP13 (USP13 Products)

Synonyms: ISOT3 antibody, IsoT-3 antibody, 2700071E21Rik antibody, AI848077 antibody, usp13 antibody, MGC163140 antibody, ubiquitin specific peptidase 13 antibody, ubiquitin specific peptidase 13 (isopeptidase T-3) antibody, ubiquitin carboxyl-terminal hydrolase 13 antibody, universal stress protein antibody, USP13 antibody, Usp13 antibody, usp13 antibody, MGYG_01656 antibody, RR_RS02895 antibody

Background:

Description: Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro, however, additional experiments are required to confirm such data.

Gene: USP13

Molecular Weight: 97 kDa

Gene ID: 8975

UniProt: Q92995

Pathways: SARS-CoV-2 Protein Interactome