Doublecortin antibody (AA 232-242)

Catalog No. ABIN625829

Product Details anti-Doublecortin Antibody

Target: Doublecortin (DCX)

Binding Specificity: AA 232-242

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This Doublecortin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: Doublecortin / DCX (aa232-242)

Sequence: KTSANMKAPQ S

Specificity: This antibody is expected to recognize all four reported isoforms (NP_000546.2, NP_835365.1, NP_835364.1, NP_001182482.1). Reported variants represent identical protein: NP_835364.1 and NP_835366.1.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: CKTSANMKAPQS

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Cerebellum shows axonal and incidental nuclear staining. Recommended concentration: 2-4 μg/mL. This antibody has been successfully used in IHC on Rat: Heysieattalab, S et al. (2021) Acta Epileptologica 3, 3

Western Blot: Approx 45 kDa band observed in Mouse fetal Brain lysates (calculated MW of 49.3 kDa according to Mouse NP_001103692.1). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 and KNRK cells. Recommended concentration: 5-10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Doublecortin

Target: Doublecortin (DCX)

Alternative Name: DCX (DCX Products)

Synonyms: DCX antibody, DBCN antibody, DC antibody, LISX antibody, SCLH antibody, XLIS antibody, Dbct antibody, 18C15.5 antibody, doublecortin antibody, DCX antibody, Dcx antibody

Background: DCX, doublecortin, RP5-914P14.1, DBCN, DC, FLJ51296, LISX, SCLH, XLIS, OTTHUMP00000023860, OTTHUMP00000216316, doublecortex, doublin, lis-X, lissencephalin-X, neuronal migration protein doublecortin

Gene ID: 1641, 13193, 84394

NCBI Accession: NP_000546, NP_835365, NP_835364, NP_001182482