MCF2L antibody (Internal Region)

Catalog No. ABIN6258277

Product Details anti-MCF2L Antibody

Target: MCF2L (MCF.2 Cell Line Derived Transforming Sequence-Like (MCF2L))

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCF2L antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: MCF2L Antibody detects endogenous levels of total MCF2L.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MCF2L, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Expiry Date: 12 months

Target Details for MCF2L

Target: MCF2L (MCF.2 Cell Line Derived Transforming Sequence-Like (MCF2L))

Alternative Name: MCF2L (MCF2L Products)

Background:

Description: Guanine nucleotide exchange factor that catalyzes guanine nucleotide exchange on RHOA and CDC42, and thereby contributes to the regulation of RHOA and CDC42 signaling pathways (By similarity). Seems to lack activity with RAC1. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity). Isoform 5 activates CDC42 (PubMed:15157669).

Gene: MCF2L

Gene ID: 23263

UniProt: O15068

Pathways: Neurotrophin Signaling Pathway, Metabolism of Steroid Hormones and Vitamin D, Thyroid Hormone Synthesis