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RORC antibody (AA 200-212)

Verified RORC Reactivity: Human WB, ELISA, FACS, IF Host: Goat Polyclonal unconjugated
Catalog No. ABIN625825
  • Target See all RORC Antibodies
    RORC (RAR-Related Orphan Receptor C (RORC))
    Binding Specificity
    • 16
    • 7
    • 7
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 200-212
    Reactivity
    • 105
    • 57
    • 23
    • 9
    • 8
    • 7
    • 6
    • 6
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 88
    • 18
    • 2
    • 1
    Goat
    Clonality
    • 90
    • 19
    Polyclonal
    Conjugate
    • 49
    • 7
    • 5
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    This RORC antibody is un-conjugated
    Application
    • 76
    • 34
    • 24
    • 23
    • 14
    • 13
    • 11
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)
    Purpose
    RORC (aa200-212)
    Sequence
    CHLEYSPERG KAE
    Specificity
    This antibody is expected to recognize both reported isoforms (NP_005051.2, NP_001001523.1).
    Cross-Reactivity
    Cow, Dog, Human, Mouse
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    CHLEYSPERGKAE
    Isotype
    IgG
    Top Product
    Discover our top product RORC Primary Antibody
  • Application Notes

    Western Blot: Approx 55-60 kDa band observed in Human Colon, Liver and Testis lysates (calculated MW of 55.8 kDa according to NP_001001523.1 and 58.2 kDa according to NP_005051.2). Recommended concentration: 0.01-0.1 μg/mL. Primary incubation 1 hour at room

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    RORC (RAR-Related Orphan Receptor C (RORC))
    Alternative Name
    RORC (RORC Products)
    Synonyms
    NR1F3 antibody, RORG antibody, RZR-GAMMA antibody, RZRG antibody, TOR antibody, Nr1f3 antibody, RORgamma antibody, Thor antibody, RAR related orphan receptor C antibody, RAR-related orphan receptor C antibody, RAR-related orphan receptor gamma antibody, RORC antibody, Rorc antibody
    Background
    RORC, RAR-related orphan receptor C, MGC129539, NR1F3, RORG, RZR-GAMMA, RZRG, TOR, RAR-related orphan receptor C, isoform a, RAR-related orphan receptor gamma, nuclear receptor ROR-gamma, nuclear receptor RZR-gamma, nuclear receptor subfamily 1 group F me
    Gene ID
    6097, 19885
    NCBI Accession
    NP_005051, NP_001001523
    Pathways
    Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
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