EHMT1 antibody (Internal Region)

Catalog No. ABIN6258149

Product Details anti-EHMT1 Antibody

Target: EHMT1 (Euchromatic Histone-Lysine N-Methyltransferase 1 (EHMT1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EHMT1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: EHMT1 Antibody detects endogenous levels of total EHMT1.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human EHMT1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Expiry Date: 12 months

Target Details for EHMT1

Target: EHMT1 (Euchromatic Histone-Lysine N-Methyltransferase 1 (EHMT1))

Alternative Name: EHMT1 (EHMT1 Products)

Synonyms: EUHMTASE1 antibody, Eu-HMTase1 antibody, FP13812 antibody, GLP antibody, GLP1 antibody, KMT1D antibody, bA188C12.1 antibody, EHMT1 antibody, G9a antibody, 9230102N17Rik antibody, D330003E03 antibody, mKIAA1876 antibody, bZ1O1.6 antibody, si:rp71-1o1.6 antibody, zgc:136244 antibody, euchromatic histone lysine methyltransferase 1 antibody, histone-lysine N-methyltransferase EHMT2 antibody, euchromatic histone-lysine N-methyltransferase 1 antibody, euchromatic histone methyltransferase 1 antibody, euchromatic histone-lysine N-methyltransferase 1a antibody, EHMT1 antibody, Ehmt1 antibody, LOC100121084 antibody, ehmt1 antibody, ehmt1a antibody

Background:

Description: Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.

Gene: EHMT1

Molecular Weight: 170 kDa

Gene ID: 79813

UniProt: Q9H9B1