CKLF antibody

Catalog No. ABIN6258091

Product Details anti-CKLF Antibody

Target: CKLF (Chemokine-Like Factor (CKLF))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CKLF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: CKLF6 Antibody detects endogenous levels of total CKLF6

Cross-Reactivity: Human, Mouse (Murine)

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide

Isotype: IgG

Application Details

Application Notes: WB 1:500~1:1000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for CKLF

Target: CKLF (Chemokine-Like Factor (CKLF))

Alternative Name: CKLF6 (CKLF Products)

Synonyms: C32 antibody, CKLF1 antibody, CKLF2 antibody, CKLF3 antibody, CKLF4 antibody, UCK-1 antibody, 1700001C14Rik antibody, 1810018M11Rik antibody, CKLF5 antibody, Cklf2 antibody, Cklf6 antibody, HSPC224 antibody, Cklf1 antibody, chemokine like factor antibody, chemokine-like factor antibody, CKLF antibody, Cklf antibody, LOC100727126 antibody, LOC101103916 antibody

Background:

Description: Master regulator of recycling and plasma membrane expression of PD-L1/CD274, an immune inhibitory ligand critical for immune tolerance to self and antitumor immunity. Associates with both constitutive and IFNG-induced PD-L1/CD274 at recycling endosomes, where it protects PD-L1/CD274 from being targeted for lysosomal degradation, likely by preventing its STUB1-mediated ubiquitination. May stabilize PD-L1/CD274 expression on antigen presenting cells and potentiates inhibitory signaling by PDCD1/CD279, its receptor on T-cells, ultimately triggering T-cell anergy.

Gene: CMTM6

Molecular Weight: 25 kDa

Gene ID: 54918

UniProt: Q9NX76