CDC42BPA antibody (Internal Region)

Catalog No. ABIN6258012

Product Details anti-CDC42BPA Antibody

Target: CDC42BPA (CDC42 Binding Protein Kinase alpha (DMPK-Like) (CDC42BPA))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC42BPA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: CDC42BPA Antibody detects endogenous levels of total CDC42BPA.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CDC42BPA, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CDC42BPA

Target: CDC42BPA (CDC42 Binding Protein Kinase alpha (DMPK-Like) (CDC42BPA))

Alternative Name: CDC42BPA (CDC42BPA Products)

Synonyms: MRCK antibody, MRCKA antibody, PK428 antibody, Mrck antibody, Pk428 antibody, A930014J19Rik antibody, DMPK-like antibody, CDC42BPA antibody, CDC42 binding protein kinase alpha antibody, GDNF family receptor alpha 2 antibody, serine/threonine-protein kinase MRCK alpha antibody, CDC42 binding protein kinase alpha (DMPK-like) antibody, CDC42BPA antibody, Cdc42bpa antibody, GFRA2 antibody, LOC100182779 antibody, LOC100545977 antibody

Background:

Description: Serine/threonine-protein kinase which is an important downstream effector of CDC42 and plays a role in the regulation of cytoskeleton reorganization and cell migration (PubMed:15723050, PubMed:9418861, PubMed:9092543). Regulates actin cytoskeletal reorganization via phosphorylation of PPP1R12C and MYL9/MLC2 (PubMed:21457715). In concert with MYO18A and LURAP1, is involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (PubMed:18854160). Phosphorylates: PPP1R12A, LIMK1 and LIMK2 (PubMed:11340065, PubMed:11399775). May play a role in TFRC-mediated iron uptake (PubMed:20188707). In concert with FAM89B/LRAP25 mediates the targeting of LIMK1 to the lamellipodium resulting in its activation and subsequent phosphorylation of CFL1 which is important for lamellipodial F-actin regulation (By similarity).

Gene: CDC42BPA

Molecular Weight: 97 kDa

Gene ID: 8476

UniProt: Q5VT25