BAF53A antibody (Internal Region)

Catalog No. ABIN6257926

Product Details anti-BAF53A Antibody

Target: BAF53A (Actin-Like 6A (BAF53A))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAF53A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: ACTL6A Antibody detects endogenous levels of total ACTL6A.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ACTL6A, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for BAF53A

Target: BAF53A (Actin-Like 6A (BAF53A))

Alternative Name: ACTL6A (BAF53A Products)

Synonyms: ACTL6 antibody, ARPN-BETA antibody, Arp4 antibody, BAF53A antibody, INO80K antibody, 2810432C06Rik antibody, AI851094 antibody, ARP4 antibody, Actl6 antibody, Baf53a antibody, C79802 antibody, baf53a antibody, fb52e06 antibody, fl26e12 antibody, chunp6900 antibody, wu:fb52e06 antibody, wu:fl26e12 antibody, actl6 antibody, MGC75671 antibody, RGD1307747 antibody, ACTL6A antibody, ATARP4 antibody, F15H18.8 antibody, F15H18_8 antibody, actin-related protein 4 antibody, Actin-like protein 4 antibody, CaO19.5623 antibody, actin antibody, actl6a antibody, actl6a.L antibody, arp4 antibody, arpn-beta antibody, ino80k antibody, actin like 6A antibody, actin-like 6A antibody, actin-related protein 4 antibody, Arp4p antibody, actin like 6A S homeolog antibody, ACTL6A antibody, Actl6a antibody, actl6a antibody, ARP4 antibody, actl6a.S antibody

Background:

Description: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Putative core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair.

Gene: ACTL6A

Molecular Weight: 47 kDa

Gene ID: 86

UniProt: O96019

Pathways: Chromatin Binding, Photoperiodism