ARHGAP18 antibody (Internal Region)

Catalog No. ABIN6257900

Product Details anti-ARHGAP18 Antibody

Target: ARHGAP18 (rho GTPase Activating Protein 18 (ARHGAP18))

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGAP18 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: RHG18 Antibody detects endogenous levels of total RHG18.

Predicted Reactivity: Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RHG18, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ARHGAP18

Target: ARHGAP18 (rho GTPase Activating Protein 18 (ARHGAP18))

Alternative Name: ARHGAP18 (ARHGAP18 Products)

Synonyms: 4833419J07Rik antibody, AI314906 antibody, AI843501 antibody, MacGAP antibody, SENEX antibody, bA307O14.2 antibody, MGC146282 antibody, DKFZp469I1011 antibody, Rho GTPase activating protein 18 antibody, Rho GTPase activating protein 18 L homeolog antibody, Arhgap18 antibody, ARHGAP18 antibody, arhgap18 antibody, arhgap18.L antibody

Background:

Description: Rho GTPase activating protein that suppresses F-actin polymerization by inhibiting Rho. Rho GTPase activating proteins act by converting Rho-type GTPases to an inactive GDP-bound state (PubMed:21865595). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts downstream of YAP1 and inhibits actin polymerization, which in turn reduces nuclear localization of YAP1 (PubMed:25778702). Regulates cell shape, spreading, and migration (PubMed:21865595).

Gene: ARHGAP18

Molecular Weight: 75 kDa

Gene ID: 93663

UniProt: Q8N392