ACOT8 antibody (Internal Region)

Catalog No. ABIN6257896

Product Details anti-ACOT8 Antibody

Target: ACOT8 (Acyl-CoA Thioesterase 8 (ACOT8))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACOT8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: ACOT8 Antibody detects endogenous levels of total ACOT8.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ACOT8, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ACOT8

Target: ACOT8 (Acyl-CoA Thioesterase 8 (ACOT8))

Alternative Name: ACOT8 (ACOT8 Products)

Synonyms: PTE1 antibody, zgc:101691 antibody, HNAACTE antibody, PTE-1 antibody, PTE-2 antibody, PTE2 antibody, hACTE-III antibody, hTE antibody, Pte1 antibody, acyl-CoA thioesterase 8 antibody, ACOT8 antibody, acot8 antibody, Acot8 antibody

Background:

Description: Acyl-coenzyme A (acyl-CoA) thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH (PubMed:9299485, PubMed:9153233, PubMed:15194431). Competes with bile acid CoA:amino acid N-acyltransferase (BAAT) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). Shows a preference for medium-length fatty acyl-CoAs (C2 to C20) (PubMed:9299485, PubMed:9153233). Inactive towards substrates with more than C20 aliphatic chains (PubMed:9153233). Involved in the metabolic regulation of peroxisome proliferation (PubMed:15194431).

Gene: ACOT8

Molecular Weight: 36 kDa

Gene ID: 10005

UniProt: O14734

Pathways: Monocarboxylic Acid Catabolic Process