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FFAR2 antibody (C-Term)

FFAR2 Reactivity: Human WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6257801
  • Target See all FFAR2 Antibodies
    FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))
    Binding Specificity
    • 16
    • 15
    • 15
    • 8
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 60
    • 19
    • 18
    • 3
    • 2
    • 1
    • 1
    Human
    Host
    • 77
    • 1
    Rabbit
    Clonality
    • 77
    • 1
    Polyclonal
    Conjugate
    • 28
    • 7
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This FFAR2 antibody is un-conjugated
    Application
    • 54
    • 29
    • 26
    • 16
    • 15
    • 13
    • 13
    • 6
    • 6
    • 5
    • 4
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    GPR43 Antibody detects endogenous levels of total GPR43.
    Purification
    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
    Immunogen
    A synthesized peptide derived from human GPR43, corresponding to a region within C-terminal amino acids.
    Isotype
    IgG
    Top Product
    Discover our top product FFAR2 Primary Antibody
  • Application Notes
    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Expiry Date
    12 months
  • Target
    FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))
    Alternative Name
    FFAR2 (FFAR2 Products)
    Synonyms
    FFA2R antibody, GPR43 antibody, Gpr43 antibody, GPCR43 antibody, free fatty acid receptor 2 antibody, FFAR2 antibody, Ffar2 antibody
    Background

    Description: G protein-coupled receptor that is activated by a major product of dietary fiber digestion, the short chain fatty acids (SCFAs), and that plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. In omnivorous mammals, the short chain fatty acids acetate, propionate and butyrate are produced primarily by the gut microbiome that metabolizes dietary fibers. SCFAs serve as a source of energy but also act as signaling molecules. That G protein-coupled receptor is probably coupled to the pertussis toxin-sensitive, G(i/o)-alpha family of G proteins but also to the Gq family (PubMed:12496283, PubMed:12711604, PubMed:23589301). Its activation results in the formation of inositol 1,4,5-trisphosphate, the mobilization of intracellular calcium, the phosphorylation of the MAPK3/ERK1 and MAPK1/ERK2 kinases and the inhibition of intracellular cAMP accumulation. May play a role in glucose homeostasis by regulating the secretion of GLP-1, in response to short-chain fatty acids accumulating in the intestine. May also regulate the production of LEP/Leptin, a hormone acting on the central nervous system to inhibit food intake. Finally, may also regulate whole-body energy homeostasis through adipogenesis regulating both differentiation and lipid storage of adipocytes. In parallel to its role in energy homeostasis, may also mediate the activation of the inflammatory and immune responses by SCFA in the intestine, regulating the rapid production of chemokines and cytokines. May also play a role in the resolution of the inflammatory response and control chemotaxis in neutrophils. In addition to SCFAs, may also be activated by the extracellular lectin FCN1 in a process leading to activation of monocytes and inducing the secretion of interleukin-8/IL-8 in response to the presence of microbes (PubMed:21037097). Among SCFAs, the fatty acids containing less than 6 carbons, the most potent activators are probably acetate, propionate and butyrate (PubMed:12496283, PubMed:12711604). Exhibits a SCFA-independent constitutive G protein-coupled receptor activity (PubMed:23066016).

    Gene: FFAR2

    Molecular Weight
    37 kDa
    Gene ID
    2867
    UniProt
    O15552
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