ARHGEF5 antibody (Internal Region)

Catalog No. ABIN6257676

Product Details anti-ARHGEF5 Antibody

Target: ARHGEF5 (rho Guanine Nucleotide Exchange Factor (GEF) 5 (ARHGEF5))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: ARHGEF5 Antibody detects endogenous levels of total ARHGEF5.

Predicted Reactivity: Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ARHGEF5, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ARHGEF5

Target: ARHGEF5 (rho Guanine Nucleotide Exchange Factor (GEF) 5 (ARHGEF5))

Alternative Name: ARHGEF5 (ARHGEF5 Products)

Synonyms: ARHGEF5 antibody, GEF5 antibody, P60 antibody, TIM antibody, TIM1 antibody, 2210412D05Rik antibody, AA717842 antibody, AW495314 antibody, Tim1 antibody, Rho guanine nucleotide exchange factor 5 antibody, rho guanine nucleotide exchange factor 5 antibody, Rho guanine nucleotide exchange factor (GEF) 5 antibody, ARHGEF5 antibody, LOC100414419 antibody, Arhgef5 antibody

Background:

Description: Guanine nucleotide exchange factor which activates Rho GTPases (PubMed:15601624). Strongly activates RHOA (PubMed:15601624). Also strongly activates RHOB, weakly activates RHOC and RHOG and shows no effect on RHOD, RHOV, RHOQ or RAC1 (By similarity). Involved in regulation of cell shape and actin cytoskeletal organization (PubMed:15601624). Plays a role in actin organization by generating a loss of actin stress fibers and the formation of membrane ruffles and filopodia (PubMed:14662653). Required for SRC-induced podosome formation (By similarity). Involved in positive regulation of immature dendritic cell migration (By similarity).

Gene: ARHGEF5

Molecular Weight: 60 kDa

Gene ID: 7984

UniProt: Q12774