ARHGEF1 antibody (N-Term)

Catalog No. ABIN6257675

Product Details anti-ARHGEF1 Antibody

Target: ARHGEF1 (rho Guanine Nucleotide Exchange Factor (GEF) 1 (ARHGEF1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: ARHGEF1 Antibody detects endogenous levels of total ARHGEF1.

Predicted Reactivity: Pig,Bovine,Sheep,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ARHGEF1, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ARHGEF1

Target: ARHGEF1 (rho Guanine Nucleotide Exchange Factor (GEF) 1 (ARHGEF1))

Alternative Name: ARHGEF1 (ARHGEF1 Products)

Synonyms: arhgef1 antibody, cb473 antibody, zgc:158261 antibody, ARHGEF1 antibody, GEF1 antibody, LBCL2 antibody, LSC antibody, P115-RHOGEF antibody, SUB1.5 antibody, Lbcl2 antibody, Lsc antibody, Rho guanine nucleotide exchange factor (GEF) 1a antibody, Rho guanine nucleotide exchange factor 1 antibody, Rho guanine nucleotide exchange factor (GEF) 1 antibody, arhgef1a antibody, ARHGEF1 antibody, arhgef1 antibody, Arhgef1 antibody

Background:

Description: Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.

Gene: ARHGEF1

Molecular Weight: 105 kDa

Gene ID: 9138

UniProt: Q92888

Pathways: Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling