CENPJ antibody (C-Term)

Catalog No. ABIN6257592

Product Details anti-CENPJ Antibody

Target: CENPJ (Centromere Protein J (CENPJ))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPJ antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: CENPJ Antibody detects endogenous levels of total CENPJ.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CENPJ, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Expiry Date: 12 months

Target Details for CENPJ

Target: CENPJ (Centromere Protein J (CENPJ))

Alternative Name: CENPJ (CENPJ Products)

Synonyms: CENPJ antibody, fi46g11 antibody, wu:fi46g11 antibody, si:dkey-46k9.9 antibody, BM032 antibody, CENP-J antibody, CPAP antibody, LAP antibody, LIP1 antibody, MCPH6 antibody, SASS4 antibody, SCKL4 antibody, Sas-4 antibody, 4932437H03Rik antibody, Gm81 antibody, centromere protein J antibody, Cenpj antibody, CENPJ antibody, cenpj antibody

Background:

Description: Plays an important role in cell division and centrosome function by participating in centriole duplication (PubMed:17681131, PubMed:20531387). Inhibits microtubule nucleation from the centrosome. Involved in the regulation of slow processive growth of centriolar microtubules. Acts as microtubule plus-end tracking protein that stabilizes centriolar microtubules and inhibits microtubule polymerization and extension from the distal ends of centrioles (PubMed:15047868, PubMed:27219064, PubMed:27306797). Required for centriole elongation and for STIL-mediated centriole amplification (PubMed:22020124). May be involved in the control of centriolar-microtubule growth by acting as a regulator of tubulin release (PubMed:27306797).

Gene: CENPJ

Molecular Weight: 149kDa

Gene ID: 55835

UniProt: Q9HC77

Pathways: M Phase