WTAP antibody (C-Term)

Catalog No. ABIN6257553

Product Details anti-WTAP Antibody

Target: WTAP (Wilms Tumor 1 Associated Protein (WTAP))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WTAP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: WTAP Antibody detects endogenous levels of total WTAP.

Predicted Reactivity: Horse,Sheep,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human WTAP, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for WTAP

Target: WTAP (Wilms Tumor 1 Associated Protein (WTAP))

Alternative Name: WTAP (WTAP Products)

Synonyms: MGC75954 antibody, WTAP antibody, RGD1563824 antibody, wu:fi29a09 antibody, zgc:66202 antibody, 2810408K05Rik antibody, 9430038B09Rik antibody, WT1 associated protein antibody, Wilms tumor 1 associated protein antibody, wilms tumor 1 associated protein antibody, WT1 associated protein L homeolog antibody, Wilms tumour 1-associating protein antibody, wtap antibody, WTAP antibody, CpipJ_CPIJ006489 antibody, Wtap antibody, wtap.L antibody

Background:

Description: Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine (m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing, processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724).

Gene: WTAP

Molecular Weight: 44 kDa

Gene ID: 9589

UniProt: Q15007