TCOF1 antibody (N-Term)

Catalog No. ABIN6257543

Product Details anti-TCOF1 Antibody

Target: TCOF1 (Treacher Collins-Franceschetti Syndrome 1 (TCOF1))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCOF1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: TCOF1 Antibody detects endogenous levels of total TCOF1.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human TCOF1, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for TCOF1

Target: TCOF1 (Treacher Collins-Franceschetti Syndrome 1 (TCOF1))

Alternative Name: TCOF1 (TCOF1 Products)

Synonyms: TCOF1 antibody, AA408847 antibody, AW209012 antibody, treacle antibody, MFD1 antibody, TCS1 antibody, treacle ribosome biogenesis factor 1 antibody, Tcof1 antibody, TCOF1 antibody

Background:

Description: Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:12777385, PubMed:26399832). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with NOLC1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832).

Gene: TCOF1

Molecular Weight: 152 kDa

Gene ID: 6949

UniProt: Q13428