RPS3 antibody (Internal Region)

Catalog No. ABIN6257472

Product Details anti-RPS3 Antibody

Target: RPS3 (Ribosomal Protein S3 (RPS3))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: RPS3 Antibody detects endogenous levels of total RPS3.

Predicted Reactivity: Pig,Zebrafish,Bovine,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RPS3, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for RPS3

Target: RPS3 (Ribosomal Protein S3 (RPS3))

Alternative Name: RPS3 (RPS3 Products)

Synonyms: S3 antibody, D7Ertd795e antibody, Rs_3 antibody, fb13d09 antibody, wu:fb13d09 antibody, zgc:56088 antibody, ribosomal protein S1a antibody, rps3 antibody, 6779 antibody, CG6779 antibody, DmRpS3 antibody, Dmel\\CG6779 antibody, M(3)124 antibody, M(3)95A antibody, M(3)B antibody, M(3)B2 antibody, M(3)Fla antibody, M(3)w antibody, M(3R)w antibody, M95A antibody, M[[w]] antibody, Mw antibody, RPS3 antibody, RS3 antibody, Rp S3 antibody, RpS antibody, Rps3 antibody, X72921 antibody, dS3 antibody, rpS3 antibody, rpls1b antibody, rpls3-b antibody, ribosomal protein S3 antibody, 40S ribosomal protein S3 antibody, ribosomal protein S3 L homeolog antibody, Ribosomal protein S3 antibody, 30S ribosomal protein S3 antibody, ribosomal protein S3 S homeolog antibody, RPS3 antibody, Rps3 antibody, rps-3 antibody, rps3 antibody, rps3.L antibody, RpS3 antibody, rps3.S antibody

Background:

Description: Involved in translation as a component of the 40S small ribosomal subunit (PubMed:8706699). Has endonuclease activity and plays a role in repair of damaged DNA (PubMed:7775413). Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA (PubMed:15707971). Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS) (PubMed:14706345). Has also been shown to bind with similar affinity to intact and damaged DNA (PubMed:18610840). Stimulates the N-glycosylase activity of the base excision protein OGG1 (PubMed:15518571). Enhances the uracil excision activity of UNG1 (PubMed:18973764). Also stimulates the cleavage of the phosphodiester backbone by APEX1 (PubMed:18973764). When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage (PubMed:23911537). Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide (PubMed:17049931). Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes (PubMed:18045535). Represses its own translation by binding to its cognate mRNA (PubMed:20217897). Binds to and protects TP53/p53 from MDM2-mediated ubiquitination (PubMed:19656744). Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization (PubMed:23131551). Involved in induction of apoptosis through its role in activation of CASP8 (PubMed:14988002). Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5 (PubMed:20605787). Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation (PubMed:22510408).

Gene: RPS3

Molecular Weight: 30 kDa

Gene ID: 6188

UniProt: P23396

Pathways: Positive Regulation of Endopeptidase Activity