NDFIP2 antibody (Internal Region)

Catalog No. ABIN6257381

Product Details anti-NDFIP2 Antibody

Target: NDFIP2 (Nedd4 Family Interacting Protein 2 (NDFIP2))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDFIP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: NDFIP2 Antibody detects endogenous levels of total NDFIP2.

Predicted Reactivity: Pig,Bovine,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human NDFIP2, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Expiry Date: 12 months

Target Details for NDFIP2

Target: NDFIP2 (Nedd4 Family Interacting Protein 2 (NDFIP2))

Alternative Name: NDFIP2 (NDFIP2 Products)

Synonyms: MGC68708 antibody, DKFZp459H0627 antibody, N4WBP5A antibody, 0710001O20Rik antibody, 2810436B12Rik antibody, 9130207N19Rik antibody, N4wbp5a antibody, mKIAA1165 antibody, Nedd4 family interacting protein 2 S homeolog antibody, Nedd4 family interacting protein 2 antibody, ndfip2.S antibody, NDFIP2 antibody, ndfip2 antibody, Ndfip2 antibody

Background:

Description: Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. Negatively regulates KCNH2 potassium channel activity by decreasing its cell-surface expression and interfering with channel maturation through recruitment of NEDD4L to the Golgi apparatus and multivesicular body where it mediates KCNH2 degradation (PubMed:26363003). May modulate EGFR signaling. Together with NDFIP1, limits the cytokine signaling and expansion of effector Th2 T-cells by promoting degradation of JAK1, probably by ITCH- and NEDD4L-mediated ubiquitination (By similarity).

Gene: NDFIP2

Molecular Weight: 36 kDa

Gene ID: 54602

UniProt: Q9NV92

Pathways: Negative Regulation of Transporter Activity, SARS-CoV-2 Protein Interactome