EIF3F antibody (Internal Region)

Catalog No. ABIN6257159

Product Details anti-EIF3F Antibody

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3F antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: EIF3F Antibody detects endogenous levels of total EIF3F.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human EIF3F, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for EIF3F

Target: EIF3F (Eukaryotic Translation Initiation Factor 3 Subunit F (EIF3F))

Alternative Name: EIF3F (EIF3F Products)

Synonyms: AO090005001590 antibody, Dmel\CG9769 antibody, eIF3-S5 antibody, eif3f antibody, LOC733116 antibody, eIF3f antibody, EIF3S5 antibody, eIF3-p47 antibody, 0610037M02Rik antibody, AA409853 antibody, Eif3s5 antibody, eukaryotic translation initiation factor 3 subunit F antibody, hypothetical protein antibody, CG9769 gene product from transcript CG9769-RA antibody, eukaryotic translation initiation factor 3 subunit 5 antibody, eukaryotic translation initiation factor 3, subunit F antibody, Eukaryotic translation initiation factor 3 subunit F antibody, eif3f antibody, ANI_1_120074 antibody, AOR_1_2780174 antibody, PTRG_03897 antibody, PAAG_00376 antibody, MCYG_08502 antibody, MGYG_08653 antibody, PGTG_04587 antibody, CG9769 antibody, LOC733116 antibody, EIF3F antibody, Eif3f antibody, eif-3.F antibody

Background:

Description: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).

Gene: EIF3F

Molecular Weight: 38 kDa

Gene ID: 8665

UniProt: O00303

Pathways: Ribonucleoprotein Complex Subunit Organization