CPEB1 antibody (Internal Region)

Catalog No. ABIN6257072

Product Details anti-CPEB1 Antibody

Target: CPEB1 (Cytoplasmic Polyadenylation Element Binding Protein 1 (CPEB1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPEB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Specificity: CPEB1 Antibody detects endogenous levels of total CPEB1.

Predicted Reactivity: Pig,Zebrafish,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CPEB1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Expiry Date: 12 months

Target Details for CPEB1

Target: CPEB1 (Cytoplasmic Polyadenylation Element Binding Protein 1 (CPEB1))

Alternative Name: CPEB1 (CPEB1 Products)

Synonyms: CEBP antibody, CPE-BP1 antibody, CPEB antibody, AU024112 antibody, Cpeb antibody, mCPEB antibody, CPEB1 antibody, cpeb1 antibody, ZOR-1 antibody, cepb1b antibody, fd15c06 antibody, wu:fd15c06 antibody, zorba antibody, cytoplasmic polyadenylation element binding protein 1 antibody, hypothetical protein antibody, cytoplasmic polyadenylation element binding protein 1 L homeolog antibody, cytoplasmic polyadenylation element binding protein 1b antibody, cytoplasmic polyadenylation element binding protein 1 S homeolog antibody, CPEB1 antibody, Cpeb1 antibody, cpeb1 antibody, LOAG_17395 antibody, cpeb1.L antibody, cpeb1b antibody, cpeb1.S antibody

Background:

Description: Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'-UTR. RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism (PubMed:24990967). In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA, a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites, those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity). Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity). Induces the assembly of stress granules in the absence of stress. Required for cell cycle progression, specifically for prophase entry (PubMed:26398195).

Gene: CPEB1

Molecular Weight: 63 kDa

Gene ID: 64506

UniProt: Q9BZB8

Pathways: Synaptic Membrane