FAK antibody (pTyr861)

Catalog No. ABIN6256813

Product Details anti-FAK Antibody

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Binding Specificity: pTyr861

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Phospho-FAK (Tyr861) Antibody detects endogenous levels of FAK only when phosphorylated at Tyrosine 861.

Predicted Reactivity: Pig,Horse,Sheep,Dog,Chicken,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human FAK around the phosphorylation site of Tyr861.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-FAK antibody (ABIN6256813)

Wu, Wang, Fang, Huang, Sun, Xiao, Yan: "MFAP5 promotes tumor progression and bone metastasis by regulating ERK/MMP signaling pathways in breast cancer." in: Biochemical and biophysical research communications, Vol. 498, Issue 3, pp. 495-501, (2018) (PubMed).

Target Details for FAK

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Alternative Name: PTK2 (PTK2 Products)

Synonyms: FADK antibody, FAK antibody, FAK1 antibody, FRNK antibody, PPP1R71 antibody, p125FAK antibody, pp125FAK antibody, PTK2 antibody, Fadk antibody, mKIAA4203 antibody, fak antibody, fak1a antibody, ptk2 antibody, ptk2a antibody, wu:fa18c04 antibody, zgc:66114 antibody, CG10023 antibody, CT28129 antibody, DFAK antibody, DFak antibody, DFak56 antibody, Dfak antibody, Dfak56 antibody, DmFAK antibody, Dmel\\CG10023 antibody, Fak56 antibody, Fak56D antibody, fak56 antibody, protein tyrosine kinase 2 antibody, protein tyrosine kinase 2 L homeolog antibody, PTK2 protein tyrosine kinase 2 antibody, protein tyrosine kinase 2ab antibody, Focal adhesion kinase antibody, PTK2 antibody, ptk2.L antibody, Ptk2 antibody, ptk2ab antibody, Fak antibody

Background:

Description: Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation, required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation, this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC, this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1, most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1, GIT2 and SHC1, this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.

Gene: PTK2

Molecular Weight: 125kDa

Gene ID: 5747

UniProt: Q05397

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling