Crk antibody (pTyr221)

Catalog No. ABIN6256674

Product Details anti-Crk Antibody

Target: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Binding Specificity: pTyr221

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Crk antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Phospho-CrkII (Tyr221) Antibody detects endogenous levels of CrkII only when phosphorylated at Tyrosine 221

Cross-Reactivity: Human, Monkey, Mouse (Murine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human CrkII around the phosphorylation site of Tyrosine 221

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for Crk

Target: Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))

Alternative Name: CrkII (CRK Products)

Background:

Description: Isoform Crk-II: Regulates cell adhesion, spreading and migration. Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.

Gene: CRK

Molecular Weight: 42kDa

Gene ID: 1398

UniProt: P46108

Pathways: Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor