BRAF antibody (pThr599)
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- Target See all BRAF Antibodies
- BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
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Binding Specificity
- pThr599
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This BRAF antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- Phospho-B Raf (Thr599) Antibody detects endogenous levels of B Raf only when phosphorylated at Threonine 599.
- Predicted Reactivity
- Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
- Purification
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
- Immunogen
- A synthesized peptide derived from human B Raf around the phosphorylation site of Thr599.
- Isotype
- IgG
- Top Product
- Discover our top product BRAF Primary Antibody
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anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibody
BRAF Reactivity: Human, Monkey WB, IHC, IF, IHC (p), FACS Host: Mouse Monoclonal 5A9 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF Reactivity: Human, Rat, Monkey, Dog WB, IHC, IF, IHC (p) Host: Mouse Monoclonal 3A7 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF Reactivity: Human, Dog WB, IHC, IHC (p) Host: Mouse Monoclonal 4C11 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 346-445) antibodyBRAF Reactivity: Human WB, ELISA, PLA Host: Mouse Monoclonal 3D2 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF Reactivity: Human ELISA, IHC Host: Mouse Monoclonal 1H12F1 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 424-453) antibodyBRAF Reactivity: Human WB, ELISA, IHC, IF, FACS Host: Rabbit Polyclonal unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF Reactivity: Human WB, ELISA, IHC, ICC Host: Mouse Monoclonal 1H12 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF Reactivity: Human, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) (pSer446) antibodyBRAF Reactivity: Human, Mouse WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF Reactivity: Human WB, FACS Host: Rabbit Polyclonal RB22681 unconjugated
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- Application Notes
- IHC 1:50-1:200, WB 1:500-2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
- Expiry Date
- 12 months
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- Target
- BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
- Alternative Name
- BRAF (BRAF Products)
- Background
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Description: Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Gene: BRAF
- Molecular Weight
- 85kDa
- Gene ID
- 673
- UniProt
- P15056
- Pathways
- MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy
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