TP53BP1 antibody (pThr543)

Catalog No. ABIN6256578

Product Details anti-TP53BP1 Antibody

Target: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Binding Specificity: pThr543

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TP53BP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Phospho-53BP1 (Thr543) Antibody detects endogenous levels of 53BP1 only when phosphorylated at Thr543

Cross-Reactivity: Human, Monkey

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Phospho-53BP1 (Thr543)

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for TP53BP1

Target: TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))

Alternative Name: 53BP1 (TP53BP1 Products)

Synonyms: TP53BP1 antibody, MGC160112 antibody, 53BP1 antibody, Tp53bp1 antibody, m53BP1 antibody, p202 antibody, Trp53bp1 antibody, tumor protein p53 binding protein 1 antibody, tumor suppressor p53-binding protein 1 antibody, tumor protein p53 binding protein 1 (predicted), 5 prime antibody, transformation related protein 53 binding protein 1 antibody, TP53BP1 antibody, tp53bp1 antibody, LOC100550605 antibody, Trp53bp1 antibody, Tp53bp1 antibody

Background:

Description: Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:22553214, PubMed:23333306, PubMed:17190600, PubMed:21144835, PubMed:28241136). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23727112, PubMed:23333306). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136). Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23760478, PubMed:28241136, PubMed:17190600). Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425). Participates to the repair and the orientation of the broken DNA ends during CSR (By similarity). In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112).

Gene: TP53BP1

Molecular Weight: 220kDa

Gene ID: 7158

UniProt: Q12888

Pathways: DNA Damage Repair