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IRAK4 antibody (pSer346, pThr345)

IRAK4 Reactivity: Human, Mouse, Rat WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6256548
  • Target See all IRAK4 Antibodies
    IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))
    Binding Specificity
    • 35
    • 16
    • 15
    • 11
    • 8
    • 8
    • 7
    • 7
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer346, pThr345
    Reactivity
    • 110
    • 63
    • 26
    • 15
    • 14
    • 12
    • 3
    • 2
    • 2
    • 2
    • 1
    Human, Mouse, Rat
    Host
    • 105
    • 10
    • 1
    Rabbit
    Clonality
    • 105
    • 11
    Polyclonal
    Conjugate
    • 48
    • 8
    • 7
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This IRAK4 antibody is un-conjugated
    Application
    • 108
    • 49
    • 37
    • 26
    • 17
    • 14
    • 11
    • 8
    • 8
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Specificity
    Phospho-IRAK4 (Thr345/Ser346) Antibody detects endogenous levels of IRAK4 only when phosphorylated at Thr345/Ser346.
    Predicted Reactivity
    Pig,Bovine,Horse,Sheep,Rabbit,Dog
    Purification
    The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
    Immunogen
    A synthesized peptide derived from human IRAK4 around the phosphorylation site of Thr345/Ser346.
    Isotype
    IgG
    Top Product
    Discover our top product IRAK4 Primary Antibody
  • Application Notes
    WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C. Stable for 12 months from date of receipt.
    Expiry Date
    12 months
  • Target
    IRAK4 (Interleukin-1 Receptor-Associated Kinase 4 (IRAK4))
    Alternative Name
    IRAK4 (IRAK4 Products)
    Synonyms
    zgc:55553 antibody, IRAK4 antibody, ipd1 antibody, irak-4 antibody, ny-ran-64 antibody, ny-ren-64 antibody, ren64 antibody, irak4 antibody, IPD1 antibody, IRAK-4 antibody, NY-REN-64 antibody, REN64 antibody, 8430405M07Rik antibody, 9330209D03Rik antibody, interleukin 1 receptor associated kinase 4 antibody, interleukin-1 receptor-associated kinase 4 antibody, IRAK4 antibody, irak4 antibody, Irak4 antibody
    Background

    Description: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.

    Gene: IRAK4

    Molecular Weight
    60 kDa
    Gene ID
    51135
    UniProt
    Q9NWZ3
    Pathways
    NF-kappaB Signaling, TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades
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