Aurora Kinase B antibody (pThr232)

Catalog No. ABIN6256516

Product Details anti-Aurora Kinase B Antibody

Target: Aurora Kinase B (AURKB)

Binding Specificity: pThr232

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora Kinase B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Phospho-AurB (Thr232) Antibody detects endogenous levels of AurB only when phosphorylated at Threonine 232

Cross-Reactivity: Human, Monkey, Mouse (Murine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human AurB around the phosphorylation site of Threonine 232

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200 IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Alternative Name: AurB (AURKB Products)

Synonyms: AIK2 antibody, AIM-1 antibody, AIM1 antibody, ARK2 antibody, AurB antibody, IPL1 antibody, PPP1R48 antibody, STK12 antibody, STK5 antibody, aurkb-sv1 antibody, aurkb-sv2 antibody, AIRK2 antibody, AL022959 antibody, Aik2 antibody, Aim1 antibody, Ark2 antibody, STK-1 antibody, Stk12 antibody, Stk5 antibody, Aur B antibody, Aurora B antibody, Aurora-B antibody, CG6620 antibody, DmAIRK2 antibody, DmAurB antibody, DmAuroraB antibody, Dmel\\CG6620 antibody, IAL antibody, IAL1 antibody, Ial antibody, aurB antibody, aurora B antibody, ARK-2 antibody, XAIRK2 antibody, aik2 antibody, aim-1 antibody, aim1 antibody, airk2 antibody, ark2 antibody, ipl1 antibody, stk12 antibody, stk5 antibody, CHUNP6896 antibody, fa09g06 antibody, stka antibody, wu:fa09g06 antibody, aurkb antibody, AIRK2-B antibody, XAIRK2-B antibody, aurkb-a antibody, aurkb-b antibody, xAurora-B-B antibody, aurora kinase B antibody, aurora B antibody, aurora kinase B S homeolog antibody, AURKB antibody, Aurkb antibody, aurB antibody, aurkb antibody, aurkb.S antibody

Background:

Description: Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (PubMed:22422861, PubMed:24814515). AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, HASPIN, and histone H3. A positive feedback loop involving HASPIN and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between HASPIN and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGO1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes.

Gene: AURKB

Molecular Weight: 39kDa

Gene ID: 9212

UniProt: Q96GD4

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades