ERK1 antibody (pThr202, pTyr204)

Catalog No. ABIN6256503

Product Details anti-ERK1 Antibody

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Binding Specificity: pThr202, pTyr204

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Phospho-ERK1/2 (Thr202/Tyr204) Antibody detects endogenous levels of ERK1/2 only when phosphorylated at Threonine 202/Tyrosine 204.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human ERK1/2 around the phosphorylation site of Thr202/Tyr204.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-ERK1 antibody (ABIN6256503)

Deng, Cheng, Wu, Wang, Zhou, Huang: "Oxabicycloheptene Sulfonate Protects Against β-Amyloid-induced Toxicity by Activation of PI3K/Akt and ERK Signaling Pathways Via GPER1 in C6 Cells." in: Neurochemical research, Vol. 42, Issue 8, pp. 2246-2256, (2018) (PubMed).

Li, Xiong, Xu, Duan, Yang, Zhou, Tu: "miR-29a regulated ER-positive breast cancer cell growth and invasion and is involved in the insulin signaling pathway." in: Oncotarget, Vol. 8, Issue 20, pp. 32566-32575, (2018) (PubMed).

Xie, Cao, Yang, Xu, Wei, Wang: "Relaxin Attenuates Contrast-Induced Human Proximal Tubular Epithelial Cell Apoptosis by Activation of the PI3K/Akt Signaling Pathway In Vitro." in: BioMed research international, Vol. 2017, pp. 2869405, (2018) (PubMed).

Peng, Wu, Deng, Zhou, Song, Yang, Zhang, Xu, Xia, Cai, Liu, Peng: "MiR-377 promotes white adipose tissue inflammation and decreases insulin sensitivity in obesity via suppression of sirtuin-1 (SIRT1)." in: Oncotarget, Vol. 8, Issue 41, pp. 70550-70563, (2018) (PubMed).

Li, Zhang, Jin, Zou, Wang, Hao, Fu, Jiao, Zhang, Lin, Matsuzaki, Zhao: "Dysifragilone A inhibits LPS‑induced RAW264.7 macrophage activation by blocking the p38 MAPK signaling pathway." in: Molecular medicine reports, Vol. 17, Issue 1, pp. 674-682, (2018) (PubMed).

Liu, Sun, Zhu, Xiao, Dong, Li: "LncRNA-TCONS_00034812 in cell proliferation and apoptosis of pulmonary artery smooth muscle cells and its mechanism." in: Journal of cellular physiology, Vol. 233, Issue 6, pp. 4801-4814, (2018) (PubMed).

Liu, Huang, Jin, Zhou, Chen, Li, Chen, Li, Yao, Li, Lan, Ye, Wang: "MicroRNA-370 inhibits the growth and metastasis of lung cancer by down-regulating epidermal growth factor receptor expression." in: Oncotarget, Vol. 8, Issue 50, pp. 88139-88151, (2018) (PubMed).

Zhang, Fei, Tao, Li, Shen, Wang, Liu, Xu: "Neuroprotective Effect of Modified Xijiao Dihuang Decoction against Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury in PC12 Cells: Involvement of TLR4-MyD88/NF-κB Signaling Pathway." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 3848595, (2018) (PubMed).

Chai, Bai, Li, Chen, Zhang: "Biological functions of lung cancer cells are suppressed in co-culture with mesenchymal stem cells isolated from umbilical cord." in: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 1076-1080, (2018) (PubMed).

Xin, Cao, Shao, Zhang, Zhang, Wang, Liang, Shao, Qi, Li, Zhang, Yang, Sun, Zhang, Jia, Wang: "Chemokine CXCL3 mediates prostate cancer cells proliferation, migration and gene expression changes in an autocrine/paracrine fashion." in: International urology and nephrology, Vol. 50, Issue 5, pp. 861-868, (2018) (PubMed).

Wu, Wang, Fang, Huang, Sun, Xiao, Yan: "MFAP5 promotes tumor progression and bone metastasis by regulating ERK/MMP signaling pathways in breast cancer." in: Biochemical and biophysical research communications, Vol. 498, Issue 3, pp. 495-501, (2018) (PubMed).

Tang, Bai, Zou, Meng, Xia, Cheng, Mu, Zhou, Wang, Qin, Cao, Jiang, Chen: "Ferroptosis is newly characterized form of neuronal cell death in response to arsenite exposure." in: Neurotoxicology, Vol. 67, pp. 27-36, (2018) (PubMed).

Huang, Song, Tao, Shao, Zeng, Xu, Qi, Sun: "Ovostatin 2 knockdown significantly inhibits the growth, migration, and tumorigenicity of cutaneous malignant melanoma cells." in: PLoS ONE, Vol. 13, Issue 4, pp. e0195610, (2018) (PubMed).

Wang, Li, Chen, Yang: "Umbilical cord‑derived mesenchymal stem cells can inhibit the biological functions of melanoma A375 cells." in: Oncology reports, Vol. 40, Issue 1, pp. 511-517, (2018) (PubMed).

Lu, Jia, Zu, Zhang, Zhao, Shi: "Inhibition of the cyclophilin A-CD147 interaction attenuates right ventricular injury and dysfunction after acute pulmonary embolism in rats." in: The Journal of biological chemistry, Vol. 293, Issue 31, pp. 12199-12208, (2018) (PubMed).

Zhang, Luan, Li, Liu, Liu, Wang, Li, Wang, Zou, Liu, Matsuzaki, Zhao: "Anti-inflammatory action of ambuic acid, a natural product isolated from the solid culture of Pestalotiopsis neglecta, through blocking ERK/JNK mitogen-activated protein kinase signaling pathway." in: Experimental and therapeutic medicine, Vol. 16, Issue 2, pp. 1538-1546, (2018) (PubMed).

Wu, Feng, Yang, Zhan, Xu, Hu, Zhu, Zhou: "Electroacupuncture exerts neuroprotective effects on ischemia/reperfusion injury in JNK knockout mice: the underlying mechanism." in: Neural regeneration research, Vol. 13, Issue 9, pp. 1594-1601, (2018) (PubMed).

Jin, Han, Yang, Hu, Liu, Zhao: "11-O-acetylcyathatriol inhibits MAPK/p38-mediated inflammation in LPS-activated RAW 264.7 macrophages and has a protective effect on ethanol-induced gastric injury." in: Molecular medicine reports, Vol. 14, Issue 1, pp. 874-80, (2017) (PubMed).

Song, Zhou, Peng, Tao, Yang, Xu, Peng, Ren, Xiang, Wei: "GPR120 promotes adipogenesis through intracellular calcium and extracellular signal-regulated kinase 1/2 signal pathway." in: Molecular and cellular endocrinology, Vol. 434, pp. 1-13, (2017) (PubMed).

Jin, Song, Wang, Zhang, Qiu, Zhao: "Tiliroside, the major component of Agrimonia pilosa Ledeb ethanol extract, inhibits MAPK/JNK/p38-mediated inflammation in lipopolysaccharide-activated RAW 264.7 macrophages." in: Experimental and therapeutic medicine, Vol. 12, Issue 1, pp. 499-505, (2017) (PubMed).

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: MAPK3,MAPK1 (MAPK3 Products)

Synonyms: ERK-1 antibody, ERK1 antibody, ERT2 antibody, HS44KDAP antibody, HUMKER1A antibody, P44ERK1 antibody, P44MAPK antibody, PRKM3 antibody, p44-ERK1 antibody, p44-MAPK antibody, Erk-1 antibody, Erk1 antibody, Ert2 antibody, Esrk1 antibody, Mnk1 antibody, Mtap2k antibody, Prkm3 antibody, p44 antibody, p44erk1 antibody, p44mapk antibody, ERK3 antibody, ERK6 antibody, P38GAMMA antibody, PRKM12 antibody, SAPK-3 antibody, SAPK3 antibody, fi06b09 antibody, wu:fi06b09 antibody, zERK1 antibody, Tb08.10J17.940 antibody, MAPK1 antibody, MNK1 antibody, AW123708 antibody, Erk6 antibody, P38gamma antibody, Prkm12 antibody, Sapk3 antibody, ATMAPK3 antibody, ATMPK3 antibody, T6D9.4 antibody, mitogen-activated protein kinase 3 antibody, mitogen-activated protein kinase 3 antibody, mitogen-activated protein kinase 12 antibody, mitogen activated protein kinase 3 antibody, mitogen-activated serine/threonine-protein kinase antibody, MAPK3 antibody, Mapk3 antibody, MAPK12 antibody, mapk3 antibody, Tc00.1047053509475.10 antibody, Tb927.8.3550 antibody, Mapk12 antibody, CEK1 antibody, MPK3 antibody

Background:

Description: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.

Gene: MAPK3

Molecular Weight: 42,44kDa

Gene ID: 5595, 5594

UniProt: P27361, P28482

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins