MED1 antibody (pThr1457)

Catalog No. ABIN6256479

Product Details anti-MED1 Antibody

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Binding Specificity: pThr1457

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Phospho-TRAP220/MED1 (Thr1457) Antibody detects endogenous levels of TRAP220/MED1 only when phosphorylated at Threonine 1457.

Predicted Reactivity: Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human TRAP220/MED1 around the phosphorylation site of Thr1457.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MED1

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Alternative Name: MED1 (MED1 Products)

Synonyms: CRSP1 antibody, CRSP200 antibody, DRIP205 antibody, DRIP230 antibody, PBP antibody, PPARBP antibody, PPARGBP antibody, RB18A antibody, TRAP220 antibody, TRIP2 antibody, CRSP-1 antibody, MED1 antibody, pbp antibody, nbr1 antibody, crsp1 antibody, rb18a antibody, trip2 antibody, pparbp antibody, crsp200 antibody, drip205 antibody, drip230 antibody, ppargbp antibody, trap220 antibody, CG7162 antibody, Dmel\\CG7162 antibody, Med1 antibody, Med1/SOP3/TRAP220 antibody, Trap220 antibody, dTRAP220 antibody, AI480703 antibody, CRSP210 antibody, Pparbp antibody, l11Jus15 antibody, RGD1559552 antibody, mediator complex subunit 1 antibody, calcitonin-related polypeptide beta antibody, Mediator complex subunit 1 antibody, mediator of RNA polymerase II transcription subunit 1 antibody, MED1 antibody, CALCB antibody, med1 antibody, Med1 antibody

Background:

Description: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (PubMed:10406464, PubMed:11867769, PubMed:12037571, PubMed:12218053, PubMed:12556447, PubMed:14636573, PubMed:15340084, PubMed:15471764, PubMed:15989967, PubMed:16574658, PubMed:9653119). Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (PubMed:24245781).

Gene: MED1

Molecular Weight: 168kDa

Gene ID: 5469

UniProt: Q15648

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha