HDAC5 antibody (pSer259)

Catalog No. ABIN6256221

Product Details anti-HDAC5 Antibody

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Binding Specificity: pSer259

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HDAC5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Phospho-HDAC5 (Ser259) Antibody detects endogenous levels of HDAC5 only when phosphorylated at Serine 259.

Predicted Reactivity: Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human HDAC5 around the phosphorylation site of Ser259.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for HDAC5

Target: HDAC5 (Histone Deacetylase 5 (HDAC5))

Alternative Name: HDAC5 (HDAC5 Products)

Synonyms: HD5 antibody, NY-CO-9 antibody, AI426555 antibody, Hdac4 antibody, mHDA1 antibody, mKIAA0600 antibody, ATHDA5 antibody, HDA5 antibody, MAF19.7 antibody, MAF19_7 antibody, histone deacetylase 5 antibody, histone deacetylase 5 antibody, HDAC5 antibody, Hdac5 antibody, HDA05 antibody, hdac5 antibody

Background:

Description: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.

Gene: HDAC5

Molecular Weight: 124kDa

Gene ID: 10014

UniProt: Q9UQL6

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Monocarboxylic Acid Catabolic Process