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ABL1 antibody (pTyr204)

ABL1 Reactivity: Human, Mouse, Rat, Monkey WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6255841
  • Target See all ABL1 Antibodies
    ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))
    Binding Specificity
    • 39
    • 36
    • 22
    • 21
    • 16
    • 15
    • 15
    • 15
    • 15
    • 15
    • 15
    • 15
    • 15
    • 14
    • 12
    • 9
    • 9
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr204
    Reactivity
    • 265
    • 145
    • 107
    • 15
    • 3
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Monkey
    Host
    • 349
    • 2
    Rabbit
    Clonality
    • 349
    • 2
    Polyclonal
    Conjugate
    • 114
    • 25
    • 24
    • 21
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    This ABL1 antibody is un-conjugated
    Application
    • 229
    • 147
    • 143
    • 143
    • 83
    • 56
    • 33
    • 31
    • 27
    • 16
    • 10
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Phospho-Abl (Tyr204) Antibody detects endogenous levels of Abl only when phosphorylated at Tyrosine 204
    Cross-Reactivity
    Human, Monkey, Mouse (Murine), Rat (Rattus)
    Purification
    The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
    Immunogen
    A synthesized peptide derived from human Abl around the phosphorylation site of Tyrosine 204
    Isotype
    IgG
    Top Product
    Discover our top product ABL1 Primary Antibody
  • Application Notes
    WB 1:500-1:2000, IF/ICC 1:100-1:500
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C.Stable for 12 months from date of receipt
    Expiry Date
    12 months
  • Target
    ABL1 (C-Abl Oncogene 1, Non-Receptor tyrosine Kinase (ABL1))
    Alternative Name
    Abl (ABL1 Products)
    Synonyms
    ABL antibody, JTK7 antibody, bcr/abl antibody, c-ABL antibody, p150 antibody, v-abl antibody, AI325092 antibody, Abl antibody, E430008G22Rik antibody, c-Abl antibody, ABL proto-oncogene 1, non-receptor tyrosine kinase antibody, c-abl oncogene 1, non-receptor tyrosine kinase antibody, Tyrosine-protein kinase abl-1 antibody, ABL1 antibody, Abl1 antibody, abl-1 antibody, abl1 antibody
    Background

    Description: Non-receptor tyrosine-protein kinase that plays a role in many key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion, receptor endocytosis, autophagy, DNA damage response and apoptosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like WASF3 (involved in branch formation), ANXA1 (involved in membrane anchoring), DBN1, DBNL, CTTN, RAPH1 and ENAH (involved in signaling), or MAPT and PXN (microtubule-binding proteins). Phosphorylation of WASF3 is critical for the stimulation of lamellipodia formation and cell migration. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as BCAR1, CRK, CRKL, DOK1, EFS or NEDD9. Phosphorylates multiple receptor tyrosine kinases and more particularly promotes endocytosis of EGFR, facilitates the formation of neuromuscular synapses through MUSK, inhibits PDGFRB-mediated chemotaxis and modulates the endocytosis of activated B-cell receptor complexes. Other substrates which are involved in endocytosis regulation are the caveolin (CAV1) and RIN1. Moreover, ABL1 regulates the CBL family of ubiquitin ligases that drive receptor down-regulation and actin remodeling. Phosphorylation of CBL leads to increased EGFR stability. Involved in late-stage autophagy by regulating positively the trafficking and function of lysosomal components. ABL1 targets to mitochondria in response to oxidative stress and thereby mediates mitochondrial dysfunction and cell death. In response to oxidative stress, phosphorylates serine/threonine kinase PRKD2 at 'Tyr-717' (PubMed:28428613). ABL1 is also translocated in the nucleus where it has DNA-binding activity and is involved in DNA-damage response and apoptosis. Many substrates are known mediators of DNA repair: DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52 or WRN. Activates the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates TP73, a primary regulator for this type of damage-induced apoptosis. Phosphorylates the caspase CASP9 on 'Tyr-153' and regulates its processing in the apoptotic response to DNA damage. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. ABL1 acts also as a regulator of multiple pathological signaling cascades during infection. Several known tyrosine-phosphorylated microbial proteins have been identified as ABL1 substrates. This is the case of A36R of Vaccinia virus, Tir (translocated intimin receptor) of pathogenic E.coli and possibly Citrobacter, CagA (cytotoxin-associated gene A) of H.pylori, or AnkA (ankyrin repeat-containing protein A) of A.phagocytophilum. Pathogens can highjack ABL1 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1. Regulates T-cell differentiation in a TBX21-dependent manner. Phosphorylates TBX21 on tyrosine residues leading to an enhancement of its transcriptional activator activity (By similarity).

    Gene: ABL1

    Molecular Weight
    135kDa
    Gene ID
    25
    UniProt
    P00519
    Pathways
    Apoptosis, Regulation of Muscle Cell Differentiation, Platelet-derived growth Factor Receptor Signaling, Lipid Metabolism
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