RPA2 antibody (pThr21)

Catalog No. ABIN6255629

Product Details anti-RPA2 Antibody

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Binding Specificity: pThr21

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Phospho-RPA32/RPA2 (Thr21) Antibody detects endogenous levels of RPA32/RPA2 only when phosphorylated at Threonine 21.

Predicted Reactivity: Pig,Zebrafish,Bovine,Sheep,Dog,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human RPA32/RPA2 around the phosphorylation site of Thr21.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Alternative Name: RPA2 (RPA2 Products)

Synonyms: REPA2 antibody, RPA32 antibody, 153194_at antibody, CG9273 antibody, Dmel\\CG9273 antibody, dRPA2 antibody, rpa antibody, zgc:109822 antibody, ik:tdsubc_2g1 antibody, xx:tdsubc_2g1 antibody, repa2 antibody, rpa32 antibody, RPA2 antibody, GB13976 antibody, LOC100230445 antibody, AA409079 antibody, AI325195 antibody, AU020965 antibody, RPA34 antibody, Rf-A2 antibody, replication protein A2 antibody, Replication protein A2 antibody, replication protein A2, 32kDa antibody, replication protein A 32 kDa subunit antibody, RPA2 antibody, Rpa2 antibody, rpa2 antibody, LOC725418 antibody, RPA2_2 antibody

Background:

Description: As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

Gene: RPA2

Molecular Weight: 35kDa

Gene ID: 6118

UniProt: P15927

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA