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MAPKAP Kinase 5 antibody (pThr182)

MAPKAPK5 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6255616
  • Target See all MAPKAP Kinase 5 (MAPKAPK5) Antibodies
    MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
    Binding Specificity
    • 26
    • 21
    • 8
    • 7
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pThr182
    Reactivity
    • 80
    • 47
    • 32
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 84
    • 9
    • 2
    Rabbit
    Clonality
    • 88
    • 7
    Polyclonal
    Conjugate
    • 40
    • 6
    • 6
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This MAPKAP Kinase 5 antibody is un-conjugated
    Application
    • 74
    • 37
    • 27
    • 27
    • 13
    • 12
    • 7
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Phospho-MAPKAPK5 (Thr182) Antibody detects endogenous levels of MAPKAPK5 only when phosphorylated at Threonine 182.
    Purification
    The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
    Immunogen
    A synthesized peptide derived from human MAPKAPK5 around the phosphorylation site of Thr182.
    Isotype
    IgG
    Top Product
    Discover our top product MAPKAPK5 Primary Antibody
  • Application Notes
    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C. Stable for 12 months from date of receipt.
    Expiry Date
    12 months
  • Target
    MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))
    Alternative Name
    MAPKAPK5 (MAPKAPK5 Products)
    Synonyms
    MAPKAPK5 antibody, MK5 antibody, PRAK antibody, MAPKAP-K5 antibody, MK-5 antibody, mitogen-activated protein kinase-activated protein kinase 5 antibody, MAP kinase-activated protein kinase 5 antibody, MAPKAPK5 antibody, mapkapk5 antibody, Mapkapk5 antibody
    Background

    Description: Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation. Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras-induced senescence and phosphorylating p53/TP53. Involved in post-transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement.

    Gene: MAPKAPK5

    Molecular Weight
    60kDa
    Gene ID
    8550
    UniProt
    Q8IW41
    Pathways
    MAPK Signaling
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