MAPT antibody (pThr181)

Catalog No. ABIN6255614

Product Details anti-MAPT Antibody

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Binding Specificity: pThr181

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: Phospho-Tau (Thr181) Antibody detects endogenous levels of Tau only when phosphorylated at Thr498, which site historically referenced as Thr181.

Predicted Reactivity: Chicken

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Tau around the phosphorylation site of Thr181.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for MAPT

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Alternative Name: MAPT (MAPT Products)

Background:

Description: Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.

Gene: MAPT

Molecular Weight: 78 kDa

Gene ID: 4137

UniProt: P10636

Pathways: MAPK Signaling, Microtubule Dynamics, M Phase, Regulation of Cell Size