Survivin antibody (pThr117)

Catalog No. ABIN6255588

Product Details anti-Survivin Antibody

Target: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Binding Specificity: pThr117

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Survivin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Phospho-Survivin (Thr117) Antibody detects endogenous levels of Survivin only when phosphorylated at Threonine 117.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Dog

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Survivin around the phosphorylation site of Thr117.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Survivin

Target: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Alternative Name: BIRC5 (BIRC5 Products)

Synonyms: AP14 antibody, API4 antibody, EPR-1 antibody, AAC-11 antibody, Api4 antibody, TIAP antibody, survivin40 antibody, bir5a antibody, survivin antibody, BIR1 antibody, Su2 antibody, XBIR1 antibody, XSurvivin1 antibody, Xsvv1 antibody, api4 antibody, bir1-A antibody, epr-1 antibody, svv1 antibody, xSurvivin antibody, xsurv antibody, Su1 antibody, birc5.2 antibody, svv2 antibody, xSurvivin2 antibody, bir5b antibody, SIX antibody, birc5.2-a antibody, birc5.2-b antibody, baculoviral IAP repeat containing 5 antibody, baculoviral IAP repeat-containing 5 antibody, baculoviral IAP repeat containing 5a antibody, baculoviral IAP repeat-containing 5, gene 1 L homeolog antibody, baculoviral IAP repeat containing 5 L homeolog antibody, baculoviral IAP repeat containing 5b antibody, baculoviral IAP repeat containing 5 S homeolog antibody, BIRC5 antibody, Birc5 antibody, birc5a antibody, birc5.1.L antibody, birc5.2.L antibody, birc5b antibody, birc5.2.S antibody

Background:

Description: Multitasking protein that has dual roles in promoting cell proliferation and preventing apoptosis (PubMed:9859993, PubMed:21364656, PubMed:20627126). Component of a chromosome passage protein complex (CPC) which is essential for chromosome alignment and segregation during mitosis and cytokinesis (PubMed:16322459). Acts as an important regulator of the localization of this complex, directs CPC movement to different locations from the inner centromere during prometaphase to midbody during cytokinesis and participates in the organization of the center spindle by associating with polymerized microtubules (PubMed:20826784). Involved in the recruitment of CPC to centromeres during early mitosis via association with histone H3 phosphorylated at 'Thr-3' (H3pT3) during mitosis (PubMed:20929775). The complex with RAN plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). May counteract a default induction of apoptosis in G2/M phase (PubMed:9859993). The acetylated form represses STAT3 transactivation of target gene promoters (PubMed:20826784). May play a role in neoplasia (PubMed:10626797). Inhibitor of CASP3 and CASP7 (PubMed:21536684). Isoform 2 and isoform 3 do not appear to play vital roles in mitosis (PubMed:12773388, PubMed:16291752). Isoform 3 shows a marked reduction in its anti-apoptotic effects when compared with the displayed wild-type isoform (PubMed:10626797).

Gene: BIRC5

Molecular Weight: 16kDa

Gene ID: 332

UniProt: O15392

Pathways: Apoptosis, Cell Division Cycle, Nuclear Hormone Receptor Binding