EIF2S1 antibody (pSer51)

Catalog No. ABIN6255389

Product Details anti-EIF2S1 Antibody

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Binding Specificity: pSer51

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2S1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Phospho-eIF2 alpha (Ser51) Antibody detects endogenous levels of eIF2 alpha only when phosphorylated at Ser52, which site historically referenced as Ser51.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human eIF2 alpha around the phosphorylation site of Ser51.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:500, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-EIF2S1 antibody (ABIN6255389)

Cai, Zheng, Gu, Wang, Wang, Yang, Zhang, Wang, Fu, Wang: "Betulinic acid chemosensitizes breast cancer by triggering ER stress-mediated apoptosis by directly targeting GRP78." in: Cell death & disease, Vol. 9, Issue 6, pp. 636, (2018) (PubMed).

Liu, Shen, Wang, Liu, Zheng, Luo: "EndophilinA2 protects against angiotensin II-induced cardiac hypertrophy by inhibiting angiotensin II type 1 receptor trafficking in neonatal rat cardiomyocytes." in: Journal of cellular biochemistry, (2018) (PubMed).

Target Details for EIF2S1

Target: EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))

Alternative Name: EIF2S1 (EIF2S1 Products)

Synonyms: EIF-2 antibody, EIF-2A antibody, EIF-2alpha antibody, EIF2 antibody, EIF2A antibody, Eif2s1 antibody, eif2s1l antibody, fb36a08 antibody, wu:fb36a08 antibody, zgc:56510 antibody, 0910001O23Rik antibody, 2410026C18Rik antibody, 35kDa antibody, Eif2a antibody, eIF2alpha antibody, eif2a antibody, CG9946 antibody, Dmel\\CG9946 antibody, Eif2alpha antibody, IF2A_DROME antibody, M(1)14C antibody, M(1)19-153 antibody, M(1)8e3-10 antibody, deIF2alpha antibody, eIF-2 antibody, eIF-2 a antibody, eIF-2 alpha antibody, eIF2 alpha antibody, eIF2-alpha antibody, eIF2a antibody, eiF-2alpha antibody, elF2alpha antibody, l(1)14Cf antibody, l(1)19-153 antibody, eukaryotic translation initiation factor 2 subunit alpha antibody, eukaryotic translation initiation factor 2, subunit 1 alpha a antibody, eukaryotic translation initiation factor 2, subunit 1 alpha antibody, eukaryotic translation initiation factor 2 alpha subunit antibody, Eukaryotic translation initiation factor 2 subunit 1 antibody, eukaryotic translation initiation factor 2 subunit alpha L homeolog antibody, eukaryotic translation Initiation Factor 2alpha antibody, EIF2S1 antibody, eif2s1a antibody, Eif2s1 antibody, eif2s1 antibody, LOC693063 antibody, CNI00230 antibody, THAPSDRAFT_105 antibody, if2a antibody, eif2s1.L antibody, eIF-2alpha antibody

Background:

Description: Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

Gene: EIF2S1

Molecular Weight: 38kDa

Gene ID: 1965

UniProt: P05198

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C