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SMAD1 antibody (pSer465)

SMAD1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6255357
  • Target See all SMAD1 Antibodies
    SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))
    Binding Specificity
    • 24
    • 24
    • 23
    • 15
    • 9
    • 8
    • 8
    • 8
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer465
    Reactivity
    • 192
    • 116
    • 87
    • 8
    • 8
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 1
    Human, Mouse, Rat
    Host
    • 192
    • 17
    Rabbit
    Clonality
    • 185
    • 25
    Polyclonal
    Conjugate
    • 98
    • 17
    • 12
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 1
    • 1
    This SMAD1 antibody is un-conjugated
    Application
    • 165
    • 80
    • 53
    • 52
    • 43
    • 28
    • 20
    • 16
    • 14
    • 12
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Phospho-Smad1 (Ser465) Antibody detects endogenous levels of Smad1 only when phosphorylated at Serine 465.
    Predicted Reactivity
    Pig,Zebrafish,Bovine,Sheep,Rabbit,Dog,Chicken,Xenopus
    Purification
    The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
    Immunogen
    A synthesized peptide derived from human Smad1 around the phosphorylation site of Ser465.
    Isotype
    IgG
    Top Product
    Discover our top product SMAD1 Primary Antibody
  • Application Notes
    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C. Stable for 12 months from date of receipt.
    Expiry Date
    12 months
  • Target
    SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))
    Alternative Name
    SMAD1 (SMAD1 Products)
    Background

    Description: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression.

    Gene: SMAD1

    Molecular Weight
    60kDa
    Gene ID
    4086
    UniProt
    Q15797
    Pathways
    Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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