ATR antibody (pSer428)

Catalog No. ABIN6255330

Product Details anti-ATR Antibody

Target: ATR (ATR serine/threonine kinase (ATR))

Binding Specificity: pSer428

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATR antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Phospho -ATR(S428) Antibody detects endogenous levels of ATR only when phosphorylated at S428

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human phospho -ATR(S428)

Isotype: IgG

Application Details

Application Notes: WB 1:1000-3000 IHC 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for ATR

Target: ATR (ATR serine/threonine kinase (ATR))

Alternative Name: ATR (ATR Products)

Synonyms: FCTCS antibody, FRP1 antibody, MEC1 antibody, SCKL antibody, SCKL1 antibody, ATR serine/threonine kinase antibody, ataxia telangiectasia and Rad3 related antibody, ATR antibody, Atr antibody

Background:

Description: Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication.

Gene: ATR

Molecular Weight: 300 kDa

Gene ID: 545

UniProt: Q13535

Pathways: Positive Regulation of Response to DNA Damage Stimulus